Journal: medRxiv

Article Title: SalivaSTAT: Direct-PCR and pooling of saliva samples collected in healthcare and community setting for SARS-CoV-2 mass surveillance

doi: 10.1101/2020.11.23.20236901

Figure Lengend Snippet: Schematic overview of sample processing and SARAS-CoV-2 assay workflow depicting main steps: Saliva samples collected in healthcare and community setting were tested and validated as follows: Upper panel: Saliva samples processed with SalivaAll protocol for nucleic acid extraction using a semi-automated instrument, followed by RT-PCR for N, ORF1ab gene targets and IC used as extraction and RT-PCR internal control; Middle panel: Saliva samples processed with SalivaSTAT method that included treatment of samples at 95° C for 30 minutes and homogenization using a bead mill homogenizer followed by direct RT-PCR; Lower panel: Saliva samples homogenized using a bead mill before pooling samples with a five-sample pooling strategy followed by SalivaSTAT method for SARS-CoV-2 testing.

Article Snippet: The SalivaSTAT method was optimized with the following conditions: saliva collected in the media-free Omni tubes was subjected to 95°C for 30 minutes followed by homogenization at 4.5 m/s for 30 seconds using the Omni bead mill homogenizer (Omni International, USA).

Techniques: Reverse Transcription Polymerase Chain Reaction, Homogenization

Journal: Cell reports

Article Title: Bordetella spp. block eosinophil recruitment to suppress the generation of early mucosal protection

doi: 10.1016/j.celrep.2023.113294

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Bead Mill Homogenizer 120V (25 mL-5mL) , VWR-Avantor , Cat#75840-022.

Techniques: Purification, Staining, Plasmid Preparation, Virus, Derivative Assay, Recombinant, Electron Microscopy, Lysis, Activation Assay, RNA Extraction, Enzyme-linked Immunosorbent Assay, Cell Isolation, Ligation, Software, Expressing, Control, Fluorescence, Microscopy, Real-time Polymerase Chain Reaction, Sequencing, Flow Cytometry, Spectrophotometry